Whole Blood Protein Extraction Kit (detergent-free)

Whole Blood Total Protein Extraction Kit is suitable for total protein extraction from a variety of animal anticoagulated/non-anticoagulated whole blood samples. The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes. The protease inhibitor mixture contained in this kit prevents the degradation of protein by protease and provides a guarantee for the extraction of high-purity protein. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel retardation experiment, and enzyme activity assay. All components of this kit do not contain detergent components, and the components of the final protein sample have no effect on downstream applications such as NI column purification, molecular sieves, ion exchange, and affinity purification. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain components that may affect mass spectrometry experiments such as SDS, Triton X-100, Chaps, etc. It does not contain detergents, high concentrations of salts, etc., and can basically meet any downstream proteomics-related experimental research. The protein extracted by this kit is an active protein with a natural protein conformation, and has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lysis time needs to be optimized according to the actual sample conditions.

This kit does not contain EDTA, therefore this kit is compatible with downstream applications such as metal chelation and chromatography.