Perl's Prussian Blue staining Kit (neutral red method)

Perl's Prussian Blue stain is often used to display various hemorrhagic lesions in local tissues, which are commonly found in phagocytes. When judging hemosiderin deposition, it can be confirmed by using the Perl's reaction. This staining method can well distinguish hemosiderin from other pigments. The dyeing solution has good stability, can be stored for a long time, is not prone to precipitation, has a wide range of applications, and can be counterstained. The counterstaining solution in this kit uses neutral red, which is a commonly used counterstaining solution.

Perl's Prussian Blue reaction is also called hemosiderin staining. It can produce Blue color after treatment with potassium ferrocyanide and dilute acid. It is common in phagocytes and in the interstitium and mainly displays ferric iron. Salt. The principle of dyeing is: the potassium ferricyanide solution causes the ferric ions to be separated from the protein by dilute hydrochloric acid. The ferric iron reacts with potassium ferricyanide to generate an insoluble Blue compound, that is, ferric iron. Ferrocyanide Prussian Blue, so this reaction is called the Prussian Blue reaction. Ferrocyanide of ferric iron is a very stable compound. After the reaction, it can be counterstained with red dyes, such as nuclear fast red, eosin, neutral red, etc.

Kit Components:
1. Perl's Stain A (50mL)
2. Perl's Stain B (50mL)
3. Neutral Red Stain (100mL)