VD2 (Vitamin D2) ELISA kit

This kit applies competitive inhibition ELISA method for the quantitative detection of VD2 (Vitamin D2) in various species samples. The microtiter plate provided in this kit has been pre-coated with VD2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of VD2 in the samples is then determined by comparing the OD of the samples to the standard curve.