Uric Acid Content Assay kit
UA is the main metabolite of birds and reptiles. The main product in normal human urine is urea with a small amount of uric acid. In addition, UA is also an important antioxidant, which can scavenge superoxide and hydroxyl radicals. The imbalance of UA production and excretion in the body can lead to the occurrence of many diseases. For example, an increase in blood UA can cause gout, renal damage and arteriosclerosis, while a decrease in UA can cause pernicious anemia, which is of great significance in clinical diagnosis.
Uricase can catalyze UA to produce allantoin, CO2 and H2O2, H2O2 oxidizes Fe2+ in potassium ferrocyanide to produce Fe3+, and Fe3+ further condenses with phenol and 4-aminoantipyrine to form red quinone compounds. There is a characteristic absorption peak at 505nm, and the content of uric acid can be calculated by measuring the absorption value at 505nm of the reaction system.
Uricase can catalyze UA to produce allantoin, CO2 and H2O2, H2O2 oxidizes Fe2+ in potassium ferrocyanide to produce Fe3+, and Fe3+ further condenses with phenol and 4-aminoantipyrine to form red quinone compounds. There is a characteristic absorption peak at 505nm, and the content of uric acid can be calculated by measuring the absorption value at 505nm of the reaction system.
Catalog No. | 250136 |
Unit Size | 50 Assays / 100 Assays |
Product Category | Enzymatic / Metabolic Assay |
Detection Method | UV Spectrophotometry |
Storage/Stability | -20°C/1 year |
Shipping | Gel Packs |