Total Cholesterol Content Assay kit
TC includes free cholesterol and cholesterol ester. TC refers to the sum of cholesterol contained in all lipoproteins in the tissue.
Use esterase to catalyze the hydrolysis of cholesterol ester to generate free cholesterol (FC) and free fatty acid (FFA), thereby converting cholesterol ester into FC; further use cholesterol oxidase to catalyze the oxidation of FC to generate 4-cholestenone and H2O2 ; Finally, peroxidase is used to catalyze H2O2 to oxidize 4-aminoantipyrine and phenol to generate red quinone compounds; there is a characteristic absorption peak at 500nm, and its color depth is proportional to the TC content.
Use esterase to catalyze the hydrolysis of cholesterol ester to generate free cholesterol (FC) and free fatty acid (FFA), thereby converting cholesterol ester into FC; further use cholesterol oxidase to catalyze the oxidation of FC to generate 4-cholestenone and H2O2 ; Finally, peroxidase is used to catalyze H2O2 to oxidize 4-aminoantipyrine and phenol to generate red quinone compounds; there is a characteristic absorption peak at 500nm, and its color depth is proportional to the TC content.
| Catalog No. | 250198 |
| Unit Size | 50 Assays / 100 Assays |
| Product Category | Enzymatic / Metabolic Assay |
| Detection Method | Visible Spectrophotometry |
| Storage/Stability | 2 ~ 8°C/1 year |
| Shipping | Gel Packs |