TBS-T [20x, pH 7.4]

TBST [20x] refers to a 20-fold concentrated stock solution of Tris-Buffered Saline with Tween-20, a common buffer solution used in molecular biology to wash away non-specifically bound antibodies and proteins. Its typical components include Tris, sodium chloride, and Tween-20, providing an isotonic and detergent-aided solution with a stable pH of approximately 7.4.