Superoxide Dismutase Activity Assay kit

SOD (EC 1.15.1.1) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the disproportionation of superoxide anions to generate H2O2 and O2. SOD is not only the superoxide anion scavenging enzyme, but also the main H2O2 generating enzyme, which plays an important role in the biological antioxidant system.

Superoxide anion (O2-.) is generated through the xanthine and xanthine oxidase reaction system, O2-. can reduce the nitrogen blue tetrazole to produce blue formazan, which absorbs at 560nm; SOD can remove O2-., thereby inhibiting the formation of formazan; the darker the blue of the reaction solution, the lower the SOD activity, and vice versa the higher the activity.