Serum (Plasma) Diamine Oxidase Activity Assay kit

DAO (Diamine oxidase,EC1.4.3.6) is widely present in animals (intestinal mucosa,lung,liver,kidney,etc.),plants and microorganisms.It catalyzes the oxidation of polyamines to aldehydes, and its activity is closely related to the synthesis of nucleic acids and proteins, which can reflect the integrity and damage of the intestinal mechanical barrier. DAO catalyzes cadaverine to produce aldehyde and hydrogen peroxide. H2O2 oxidizes Fe2+ in potassium ferrocyanide to form Fe3+. Fe3+ further reacts with 4-aminoantipyrine and phenol to form red quinone compounds. There is a characteristic absorption peak at 505 nm. The activity of DAO is calculated by measuring the absorbance at 505 nm.