Pyruvate Kinase Activity Assay kit

PK (EC 2.7.1.40) widely exists in animals, plants, microorganisms and cultured cells. It catalyzes the last reaction in the glycolysis process and is one of the main rate-limiting enzymes in the glycolysis process, and it is also produced One of the key enzymes of ATP, so the determination of PK activity is of great significance.

PK catalyzes phosphoenolpyruvate and ADP to generate ATP and pyruvate, and lactate dehydrogenase further catalyzes NADH and pyruvate to generate lactic acid and NAD+. The decrease rate of NADH can be measured at 340nm to reflect PK activity.