Pyrroline-5-Carboxylate Reductase Activity Assay kit

Pyrroline-5-carboxylate reductase (P5CR) is an important housekeeping protein widelyfoundin prokaryotes and eukaryotes. In the presence of NAD(P)H, pyrroline-5-carboxylic acid(P5C)isconverted to proline by pyrroline-5-carboxylate reductase, and P5CR has also been foundtobeinvolved in the metabolism of thioproline in E. coli. Thioproline is dehydrogenated by pyrroline-5-carboxylate reductase and is accompaniedbytheconversion of NAD to NADH. In the presence of 1-mPMS, WST-1 reacts with NADHtoproducewater-soluble formazan with a characteristic absorption peak at 450 nm.