Protein Disulfide Bond Content Assay kit

Proteins are an important class of biomolecules found in all living organisms andarethebasicsubstances of life. Disulfide bond is a chemical bond that connects the sulfhydryl groups of twodifferentcysteine residues in different peptide chains or the same peptide chain. Disulfide bonds haveagreatinfluence on the structure of proteins and play a role in stabilizing the spatial structure of peptidechainsinprotein molecules, so it is particularly important to determine the content of protein disulfide bonds. Reducing agent will make the disulfide bond cleaved, the cleaved sulfhydryl groupwill undergonucleophilic reaction, that is, the sulfhydryl group reacts with 5,5'-dithio-bis-nitrobenzoic acid(DTNB)toproduce a yellow color compound, with a maximum absorption peak at 412 nm, accordingtowhich,wecan calculate the content of the disulfide bond of the protein.