Phosphoenolpyruvate Carboxylase Activity Assay kit

PEPC (EC 4.1.1.31) is widely present in animals, plants, microorganisms and cells. It is an enzyme that catalyzes the reaction of phosphoenolpyruvate with carbon dioxide to produce oxaloacetate, which is an irreversible reaction to the tricarboxylic acid cycle. Play an important regulatory role.

PEPC catalyzes phosphoenolpyruvate and CO2 to produce oxaloacetate and HPO42-, and malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+. The NADH reduction rate is measured at 340nm and the PEPC activity is calculated.