NP-40 Lysis Buffer
NP-40 Lysis Buffer is a relatively mild cell and tissue lysate. The protein sample obtained by lysis of NP-40 lysate can be used for Conventional PAGE, Western, immunoprecipitation (immunol precipitation, IP), co-immunoprecipitation (co-IP) and ELISA, etc. This product can be used for animal and plant cell or tissue samples, as well as for fungal or bacterial samples.
The main components of NP-40 lysis buffer are Tris (pH 7.4), NaCl, 1% NP-40, EDTA and phosphatase inhibitor. In addition, this product also presents a PMSF, which can effectively inhibit protein degradation.
The protein sample obtained by lysing with NP-40 Lysis Buffer can be used to determine the protein concentration with the BCA protein concentration determination kit. Due to Containing a higher concentration of detergent, the Bradford method cannot be used to determine the protein concentration of the sample lysed from this lysate.
The main components of NP-40 lysis buffer are Tris (pH 7.4), NaCl, 1% NP-40, EDTA and phosphatase inhibitor. In addition, this product also presents a PMSF, which can effectively inhibit protein degradation.
The protein sample obtained by lysing with NP-40 Lysis Buffer can be used to determine the protein concentration with the BCA protein concentration determination kit. Due to Containing a higher concentration of detergent, the Bradford method cannot be used to determine the protein concentration of the sample lysed from this lysate.
| Catalog No. | 501002 |
| Unit Size | 100mL |
| Product Category | Protein Isolation |
| Storage/Stability | -20°C/1 year |
| Shipping | Ambient |