Nitric Oxide Synthase Activity Assay kit

Nitric Oxide Synthase (NOS, EC 1.14.13.39) is a kind of enzyme that catalyzes the synthesisof NO from L-arginine in vivo. It mainly exists in vascular smooth muscle cells, macrophages,endothelial cells, nerve cells, liver cells, glomerular membrane cells and other cells. As a cell signalmolecule, it plays a very important role in the nervous, immune and cardiovascular systemsofthebody.NOS catalyzes L-arginine, molecular oxygen and NADPH to form NOand NADP+. NOiseasily oxidized to form NO2- and NO3- in aqueous solution. Under acidic conditions, NO2- anddiazonium sulfonamide produce diazo compounds. The compounds could further couplewithnaphthyl vinyl diamine. The product has a characteristic absorption peak at 550 nmanditsabsorbance value can be measured to calculate NOS activity.