Nitrate Reductase Assay kit (Griess method)

NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen intoammonianitrogen as well as an induction enzyme, which widely exists in plants and has an impact oncropyield and quality. NR catalyzed nitrate reduction to nitrite, with NO3? +NADH+H? ?NO2?+NAD? +H2O. Intheacidic conditions, the NO2? produced can participate in the diazotization reaction toproduceapurple-red compound, which has an absorption peak at 540 nm, and the change in absorbancevalueat 540 nm indicates the enzyme activity.