Methylcitrate Synthase Activity Assay kit

Methylcitrate Synthase (MCS) is widely present in the mitochondrial matrix of animals, plants,microorganisms and cultured cells, and is involved in the regulation of tricarboxylic acidcycletogether with citrate synthase (CS). MCS catalyzes propionyl CoA and oxaloacetic acid to produce methyllimonyl CoenzymeA, which further hydrolyzes to produce methylcitric acid. The reaction transforms colorless DTNBinto yellow TNB with a characteristic absorption peak at 412nm, from which MCS activitycanbecalculated.