Malic Acid Content Assay kit

L-malic acid is an intermediate product of the tricarboxylic acid cycle and an important part ofthe malate-aspartic acid shuttle. Malate-aspartic acid shuttle is required for the reductive equivalenttransmitochondrial membrane transport process of oxidative phosphorylation. In lower organisms,malic acid is converted to lactic acid in the process of malolactic fermentation, which alsoproducesCO2. Malic acid is commonly used as an additive in the food and pharmaceutical industries, andquantitative analysis of malic acid is also vital in the production of beer, wine, cheese andfruit. Malate dehydrogenase catalyzes malic acid and NAD to form oxaloacetic acid, NADH and NH4+. Under 1-mPMS, WST-1 reacts with NADH to produce water-soluble Formazan. Themaximum absorption peak at 450nm can be used to calculate malic acid content.