Kanamycin [100mg/mL]
X-gal is a chromogenic substrate, used for blue and white spot screening.
IPTG and X-gal combined, are used for blue and white spot screening. IPTG can induce the vector Lac operon DNA segment to synthesize the amino terminal fragment of beta-galactosidase, which can achieve intragenic complementation (alpha complementation) with the defective beta-galactosidase encoded by the host cell. Bacteria that achieve alpha complementation can be formed on X-gal chromogenic substrates to form blue colonies. Insertion of foreign DNA into the multiple cloning site of the plasmid can disrupt alpha complementation and produce white colonies.
This product is a solution of X-gal dissolved in DMF.
IPTG and X-gal combined, are used for blue and white spot screening. IPTG can induce the vector Lac operon DNA segment to synthesize the amino terminal fragment of beta-galactosidase, which can achieve intragenic complementation (alpha complementation) with the defective beta-galactosidase encoded by the host cell. Bacteria that achieve alpha complementation can be formed on X-gal chromogenic substrates to form blue colonies. Insertion of foreign DNA into the multiple cloning site of the plasmid can disrupt alpha complementation and produce white colonies.
This product is a solution of X-gal dissolved in DMF.
| Catalog No. | 515013 |
| Unit Size | 5mL |
| Product Category | Cloning / Ligation |
| Storage/Stability | -20°C/1 year |
| Shipping | Gel Packs |