Isocitrate Dehydrogenase Cytoplasmic Activity Assay kit

ICDHc (EC 1.1.1.42) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the dehydrogenation and decarboxylation of isocitrate to produce alpha-ketoglutarate. Restore NADP at the same time. Generate NADPH+. ICDHc is another important source of NADPH in the cytoplasm besides the pentose phosphate pathway. In adversity, the enzyme activity usually changes significantly.

Use ICDHc to catalyze NADP+ Reduction to NADPH reaction, measuring the increase of NADPH concentration at 340nm can reflect the activity of ICDHc.