IgG Purification kit (Protein A)

The IgG Purification kit is useful for routine purification of antibodies. Samples containing IgG are incubated with immobilized Protein A in a buffer that facilitates binding. After non-IgG components are washed from the spin column, the bound IgG is recovered by elution. The supplied buffers provide for maximum immunoglobulin binding and elution efficiency with the Protein A column. Each 3mL colum contains 2mL of Protein A agrose beads, and each column can purify up to 25mg of IgG.

1. Protein A 4FF column [2mL]: 2 units / 5 units;
2. IgG Binding Buffer [10x]: 10mL / 50mL;
3. IgG Elution Buffer [1x]: 25mL / 100mL;
4. Neutralization Buffer [1x]: 10mL / 25mL