Human MAP2K1 (Mitogen Activated Protein Kinase Kinase 1) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of MAP2K1 (Mitogen Activated Protein Kinase Kinase 1) in human samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP2K1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP2K1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP2K1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human MAP2K1 in the samples is then determined by comparing the OD of the samples to the standard curve.