Glycosylated Hemoglobin Content Assay kit

Glycated hemoglobin (GHb) is formed when a non-enzyme reaction occurs betweenglucoseinthe serum and hemoglobin in the erythrocyte. The amount of GHb generated is proportional tothemean blood glucose during the 8–10 weeks before the test. GHb is composed of HbA1a, HbA1band HbA1c. HbA1c accounts for about 70% and its structure is relatively stable. GHbcontent isused as a biomarker for diabetes mellitus control. The hexose moiety of GHb is converted to 5-hydroxymethylfurfural (5-HMF) byheatingat100? in the presence of a weak acid. 5-HMF reacts with Thiobarbituric Acid (TBA) toproducecolored compound, which has an absorption peak at 443 nm.