Glycogen Phosphorylase b Activity Assay kit

Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogenphosphorylase a, GPa) and inactive glycogen phosphorylase b (Glycogen phosphorylaseb, GPb)two forms. The decomposition of glycogen is mainly carried out under the catalysis of glycogenphosphorylase a. When no activator is added, glycogen phosphorylase a catalyzes the production of glucose residuesfromglycogen and inorganic phosphorus to glycogen and glucose 1-phosphate. Under the actionofphosphoglucose mutase and 6-phosphate glucose dehydrogenase, it further catalyzes the reductionof NADP to NADPH. Measuring the rate of increase of NADPH at 340nm can reflect the activityofglycogen phosphorylase a. The activity of GP (GPa and GPb) is measured by adding anactivator,and GPa activity is measured when no activator is added. GPb activity can be obtainedbysubtracting GPa activity from GP activity.