Glutamine Synthetase Activity Assay kit

GS (EC 6.3.1.2) mainly exists in plants and is one of the key enzymes of ammonia assimilation in organisms. It catalyzes the synthesis of glutamine from ammonium ions and glutamate, which can not only prevent excessive ammonium ions from affecting organisms. Toxicity, and glutamine is also the main form of ammonia storage and transportation.

GS catalyzes the synthesis of glutamine from ammonium ion and glutamate in the presence of ATP and Mg2+; glutamine is further converted to gamma-glutamyl hydroxamic acid, which forms a red complex with iron under acidic conditions The complex has a maximum absorption peak at 540nm, which can be measured with a spectrophotometer.