gamma-Glutamylcysteine Ligase Activity Assay kit
gamma-Glutamylcysteine Ligase (GCL) is the rate-limiting enzyme of GSH synthesis, and GSH has a feedback inhibitory effect on GCL. GCL gene expression is regulated by many factors, such as oxidants, antioxidants, growth factors and inflammatory factors. The activity of GCL has an important influence on the content of GSH and the ratio of GSH/GSSG.
In the presence of ATP and Mg2+, GCL catalyzes the synthesis of gamma-glutamylcysteine from glutamic acid and cysteine; at the same time, ATP dephosphorylates to produce inorganic phosphorus molecules. By measuring the increase rate of inorganic phosphorus, you can Calculate GCL activity.
In the presence of ATP and Mg2+, GCL catalyzes the synthesis of gamma-glutamylcysteine from glutamic acid and cysteine; at the same time, ATP dephosphorylates to produce inorganic phosphorus molecules. By measuring the increase rate of inorganic phosphorus, you can Calculate GCL activity.
| Catalog No. | 250121 |
| Unit Size | 50 Assays / 100 Assays |
| Product Category | Enzymatic / Metabolic Assay |
| Detection Method | Visible spectrophotometry |
| Storage/Stability | -20°C/1 year |
| Shipping | Gel Packs |