Fluoride Resistant Acid Phosphatase Activity Assay kit

Fluoride resistant acid phosphatase (FRAP) is a type of acid phosphatase. FRAPis mainlyfound in the lysosomes of most cells, the prostate gland, brain, liver, spleen, and platelets. The activity of fluoride-resistant acid phosphatase is not inhibited by fluoride ions, whereastheactivity of other acid phosphatases is inhibited by fluoride ions. Under acidic conditions, thefluoride-resistant ion acid phosphatase catalyzes the production of p-nitrophenol fromPNPP. Thep-nitrophenol appears yellow under alkaline conditions and can be detected at 400 nmabsorbance.The darker the yellow color of the product, the higher the activity of the fluorine-resistant ionacidphosphatase, and vice versa, the lower the enzyme activity