Endotoxin-Free Plasmid DNA Extraction Maxi Kit

The Kit is designed to lysis the cell by alkali pyrolysis, then to specifically adsorb DNA using a centrifugal adsorption column in the state of high-salt. Endotoxin scavenging agent can efficiently clean out endotoxin from the plasmid solution. Purified plasmid contains endotoxin less than 0.1EU/ug. The silicon matrix of centrifugal adsorption column can efficiently and exclusively adsorb DNA and exclude the protein of impurities and other organic compound.200-300ug pure and high copies of plasmid DNA can be fast extracted from 50-100 ml LB (Luria-Bertani), and the extraction rates peek to 85-90 %. Acquired plasmid DNA using our kit is available for a broad range of routine operation, including enzyme-cut, PCR, sequencing, linking and transformation.

The Endotoxin-Free Plasmid DNA could be used for a broad range of routine application, including enzyme digestion, PCR, library construction, Southern Blotting etc.