EdU Cell Proliferation Assay kit (imaging assay)

EdU (5-Ethynyl-2'-deoxyuridine) is a thymidine analog capable of replacing the thymus during cell proliferation Pyrimidine (T) is incorporated into replicating DNA molecules and rapidly detected by specific reaction based on EdU and Apollo fluorescent dyes. Cell DNA replication activity, which can quickly and accurately detect the proliferation ability of cells. Compared with the BrdU detection method, the EdU detection method is more Faster, more responsive and more accurate. EdU is very similar to T, and EdU dyes are only 1/500 of that of BrdU antibodies and are easily Easy diffusion, no DNA denaturation (acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, etc.), which can effectively avoid sample damage, and does not require antigen-antibody reaction, It can more accurately reflect DNA replication activity at the cellular and tissue level.

This kit is suitable for the proliferation detection of cells cultured in vitro. Adherent cells shouLd be detected by fluorescence, which is suitable for fluorescence microscopy, Confocal microscopy, high content screening instrument detection. Suspension cells can be smeared after incubation with EdU, from fixation to adherence after smearing Cells were detected using the same staining procedure.