D-lactate Dehydrogenase Activity Assay kit

Lactate dehydrogenase (LDH) is the terminal enzyme of the glycolysis pathwaywhichiswidely found in animals, plants, microorganisms and cultured cells. LDH catalyzes the reversibleconversion of lactate to pyruvic acid with the reduction of NAD+ to NADHand viceversa.According to the different configuration of catalytic substrate, it could be divided intoD-lactatedehydrogenase (D-LDH, EC1.1.1.28) and L-lactate dehydrogenase (L-LDH, EC1.1.1.27). NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of D-LDH. Pyruvatefurtherreacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which showbrownredcolor in alkaline solution and the color depth is proportional to the concentration of pyruvate.