Coenzyme II NADPH Content Assay kit (WST method)

Coenzyme II NADP(H) is widely present in animals, plants, microorganisms and culturedcells.The determination of NADP+ and NADPH content can calculate the content of NADP(NADPH+NADP+) and the ratio of NADPH/NADP+, and its changes are related to the pentose phosphatepathway and biosynthesis and Antioxidative responses are closely related. The NADPH/NADP+ratio is not only one of the main markers of cellular redox state, but also plays animportantregulatory role in the PPP pathway, biosynthesis and antioxidant metabolism. The NADP+ and NADPH in the samples were extracted with acidic and basic extractionsolutions, respectively. Under the action of 1-mPMS, WST-1 can react with NADPHtoproducewater-soluble formazan, which has a characteristic absorption peak at 450nm, while NADP+ canbereduced to NADPH by 6-phosphate glucose dehydrogenase, which is further detected by WST-1.