Chelating Beads 6FF

Chelating Beads 6FF (Fast Flow) are based on a highly cross-linked 6% agarose gel matrix, and the ligand is iminodiacetic acid (IDA). Chelating Beads 6FF can be used to chelate various metal ions, such as Cu 2+, Zn 2+, Co 2+, Ni 2+ and Fe 3+, which can be selected according to the binding ability of metal ions to tagged proteins. Metal ion. Ni 2+ is usually preferred because the nickel ion chelating filler allows for one-step purification of his-tagged proteins from a variety of expression systems. Cu 2+, Zn 2+, with strong binding force, can be used to purify proteins with weak binding force. Co 2+ and Fe 3+ are more specific to tagged proteins and have higher purity.