Bst II DNA Polymerase (Large Fragment, Glycerol Free)

Bst II DNA Polymerase (Large Fragment, Glycerol Free) is a modified version of Bacillus stearothermophilus DNA polymerase. It has been engineered and expressed in Escherichia coli. This product exhibits strong 5-3' DNA polymerase activity and strand displacement activity but lacks 5-3' exonuclease activity, and the optimal reaction temperature is 65?. lt is suitable for various isothermal amplification reactions, such as Loop-Mediated lsothermal Amplification (LAMP), Rolling Circle Amplification (RCA), Helicase-Dependent Amplification (HDA), etc.

Compared to the wild-type enzyme, Bst II DNAPolymerase (Large Fragment, Glycerol Free) has a faster amplification rate, stronger dUTP tolerance, salt tolerance, and improved stability. 

This product is glycerol-free and can be used for the preparation of various lyophilized reagents.

Component supplied: 1. Bst II DNA Polymerase (Large Fragment, Glycesrol Free): 200uL / 1mL
2. Bst II Reaction Buffer [10x]: 1mL / 3 x 1mL
3. MgSO? [100 mM]: 1mL / 3 x 1mL