Bradford Protein Assay

Bradford Protein Assay kit uses Coomassie Brilliant Blue G-250 dye to determine the protein concentration. The Coomassie G-250 dye, combined with protein in an acidic solution, makes the position of the maximum absorption peak of the dye from 465nm to 595nm. Within a certain concentration range, the measured absorbance value A595 and protein concentration are just proportional.

The reagents supplied in the kit couLd conduct the 2500 micro-assays.

Note: the Bradford method determines that the protein concentration is not affected by the chemicals in most samples. The concentration of beta-mercaptoethanol in the sample can be as high as 1M, and the concentration of dithiothreitol (DTT) can be as high as 5mM, but it is affected by a slightly higher concentration of detergent. It is necessary to ensure that the concentration of SDS is less than 0.1%, Triton X-100 is less than 0.1%,, and Tween-20/60/80 is less than 0.06%.


The samples containing detergents are recommended to use the BCA Protein Assay Kit (Cat. No. 210001).

Kit components:
1) 5x G250 Staining solution: 100mL
2) PBS Diluent: 30mL
3) Protein Standard (5mg/mL BSA): 1mL