ATP Content Assay kit (WST-1 method)

ATP (adenosine 5'-triphosphate) is found broadly in animals, plants, microorganismsandcultured cells, which is described as the energy currency in all living systems. Detectingthecontentof ATP and calculating the level of energy charge can reflect the state of energy metabolism. Hexokinase (HK) catalyzes the synthesis of glucose and ATP into 6-phosphate glucose.6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of glucose 6-phosphateand NADP to form NADPH. NADPH has a characteristic absorption peak at 340nm, the absorbance ratio of NADPH is in direct proportion to contents of ATP