Ascorbic Acid / Total Ascorbic Acid Assay kit

Ascorbic acid (AsA) is a kind of coenzyme, free radical scavenger, electron acceptor andsubstrateof oxalate and tartrate biosynthesis. As the most important antioxidant in plant cells, AsAplaysanimportant role in protecting chloroplasts from oxidative damage, and is also one of the importantindicators to measure the quality of crop products. Dehydroascorbic acid (DHA) is a reversibleoxidation type of AsA. In vivo, it forms an redox system with ascorbic acid and has the functionofelectron acceptor. AsA is reductive and can reduce Fe3+ to Fe2+. Fe2+ and 2,2'-bipyridine forma pink complexwithacharacteristic absorption peak at 525 nm. DTT can reduce DHA to produce AsA, which canbeusedto detect the content of total ascorbic acid (AsA+DHA) in samples.