AR Protein A 4FF column
AR Protein A 4FF is an alkali-resistant affinity chromatography medium for the separation and purification of monoclonal antibodies, polyclonal antibodies or Fc-fusion proteins.
Protein A is a cell wall protein isolated from Staphylococcus aureus that binds mammalian IgG mainly through an Fc fragment. The ligand protein for AR Protein A 4FF is an alkali-resistant protein A (so abbreviated as AR) obtained by bioengineering mutation of recombinant protein A, expressed in E.coli. There are no animal-derived components are used in the ligand purification process. The ligands are specially designed for enhanced stability to alkalis and proteases.
AR Protein A is based on a highly cross-linked 4% agarose gel, which can purify monoclonal and polyclonal antibodies at relatively high flow rates. Its pressure resistance makes it suitable for use in Large-scale production of industrialized antibodies.
While it is cleaned with 0.1M NaOH for 200 times; the medium load remain almost unchanged. After cleaning with 0.5M NaOH for 100 times, the load can still reach 80% of the initial load. It uses a more stable directional coupling with low shedding (less than 10ng/mg IgG).
Protein A is a cell wall protein isolated from Staphylococcus aureus that binds mammalian IgG mainly through an Fc fragment. The ligand protein for AR Protein A 4FF is an alkali-resistant protein A (so abbreviated as AR) obtained by bioengineering mutation of recombinant protein A, expressed in E.coli. There are no animal-derived components are used in the ligand purification process. The ligands are specially designed for enhanced stability to alkalis and proteases.
AR Protein A is based on a highly cross-linked 4% agarose gel, which can purify monoclonal and polyclonal antibodies at relatively high flow rates. Its pressure resistance makes it suitable for use in Large-scale production of industrialized antibodies.
While it is cleaned with 0.1M NaOH for 200 times; the medium load remain almost unchanged. After cleaning with 0.5M NaOH for 100 times, the load can still reach 80% of the initial load. It uses a more stable directional coupling with low shedding (less than 10ng/mg IgG).
| Catalog No. | 506052 |
| Unit Size | 5 x 1mL / 1 x 5mL / 5 x 5mL |
| Product Category | Affinity Purification |
| Matrix | Highly Cross-Linked 4% Agarose Beads |
| Ligand | Alkali Resistant Protein A |
| Chemical Resistance | Tolerates all reagents |
| Binding Capacity | >40mg human lgG/ml |
| Bead Size | ~90um |
| Flow Velocity | 0-300 cm/h |
| pH Stability | 3-12 |
| Cleaning In-Place | 0.1-0.5M NaOH |
| Storage Buffer | 1x PBS containing 20% Ethanol |
| Storage/Stability | 2-8°C/2 years |
| Shipping | Gel Packs |