alpha-Ketoglutarate Dehydrogenase Activity Assay kit

alpha-KGDH (EC1.2.4.2) is widely present in the mitochondria of animals, plant microorganisms and cultured cells. It is one of the key enzymes in the regulation of the tricarboxylic acid cycle and catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl Coenzyme A.

alpha-KGDH catalyzes alpha-ketoglutarate, NAD+ and coenzyme A to produce succinyl-CoA, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340nm, and the rate of NADH production represents alpha-KGDH activity.