Acetoacetate Content Assay kit

Acetoacetic acid (AcAc) is one of the important components of ketone body. It is about 20%ofthetotal ketone body in normal people. It is a strong organic acid produced by oxidation of fattyacids.Normal content of acetoacetic acid is harmless to human body. In diabetic patients, the amount ofacetoacetic acid is accumulated due to the decrease of carbohydrate use or starvationduetothemetabolic disorder of sugar. Acetoacetic acid can be converted into acetone as well as acetonebeta-Hydroxybutyric acid. At pH 7.0 and 37?, beta- Hydroxybutyrate dehydrogenase (HBDH) reducedAcActo3-hydroxybutyrate or decarboxylated to acetone, and NADH was oxidized to NAD+. In the presenceof 1-mPMS, WST-1 can react with NADH to produce water-soluble formazan with a characteristicabsorption peak at 450nm. The content of AcAc can be calculated by detecting the wavelengthchange at 450nm.