Rat p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) in rat samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.