Rat IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of IL-2sRa/CD25 (Soluble Interleukin-2 Receptor alpha chain) in rat samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.