Porcine TNFb (Tumor Necrosis Factor beta) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of TNFb (Tumor Necrosis Factor beta) in porcine samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TNFb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TNFb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TNFb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Pig TNFb in the samples is then determined by comparing the OD of the samples to the standard curve.