Porcine F1+2 (Prothrombin Fragment 1+2) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of F1+2 (Prothrombin Fragment 1+2) in porcine samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Pig F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.