Porcine C5a (Complement Component 5a) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of C5a (Complement Component 5a) in porcine samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Pig C5a in the samples is then determined by comparing the OD of the samples to the standard curve.