Normal Chicken serum
Normal Chicken serum as a blocking agent or antibody diluent in immunoassays to reduce non-specific binding in techniques like ELISA and immunohistochemistry. It also serves as a negative control or reference in various immunological & serological studies.
While using, dilute the blocking serum stock solution 10 or 20 times with PBS (0.01mol/L pH7.2-7.4) to make a 10% or 5% working solution, and drop it directly on the tissue slice or cell smear Incubate at 37°C for 10-30 minutes, remove the serum, do not wash, add dropwise primary antibody working solution diluted in appropriate proportions, incubate at 37°C for 1-2h or 4°C overnight.
While using, dilute the blocking serum stock solution 10 or 20 times with PBS (0.01mol/L pH7.2-7.4) to make a 10% or 5% working solution, and drop it directly on the tissue slice or cell smear Incubate at 37°C for 10-30 minutes, remove the serum, do not wash, add dropwise primary antibody working solution diluted in appropriate proportions, incubate at 37°C for 1-2h or 4°C overnight.
Catalog No. | 506101 |
Unit Size | 10mL |
Product Category | Immunoreagent |
Storage/Stability | -20°C/5 years |
Shipping | Gel Packs |