Mouse TSG101 (Tumor Susceptibility Gene 101) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of TSG101 (Tumor Susceptibility Gene 101) in mouse samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TSG101. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TSG101. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TSG101, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse TSG101 in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No.3029041
Unit Size 96-Wells
Product CategoryELISA (Quantitative)
Reactivity Mouse
Sample Serum; Plasma; Tissue Homogenates; Other Biological Fluids
Assay Method Sandwich ELISA
Assay Duration 3.5 hours
Sensitivity 0.114 ng/mL
Standard Curve Range 0.32-20 ng/mL
Storage/Stability -20°C/1 year; 4°C/6 months

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