Human Anti-NMDAR (Anti-N-methyl-D-aspartic Acid Receptor Antibody) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of Anti-NMDAR (Anti-N-methyl-D-aspartic Acid Receptor Antibody) in human samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-NMDAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-NMDAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-NMDAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human Anti-NMDAR in the samples is then determined by comparing the OD of the samples to the standard curve.