Human a1ACT (alpha-1-Antichymotrypsin) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of a1ACT (alpha-1-Antichymotrypsin) in human samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a1ACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human a1ACT in the samples is then determined by comparing the OD of the samples to the standard curve.