HRP

Horseradish peroxidase (HRP) is a versatile enzyme isolated from horseradish roots, widely used in diagnostics and biochemistry for its ability to oxidize various organic substrates using hydrogen peroxide. HRP serves as a signal amplifier in techniques like ELISA, immunohistochemistry, and Western blots, and is employed in neuroscience as a retrograde tracer. Its catalytic activity is based on the formation of stable oxidized intermediates during the reaction with peroxide, which can be detected using chromogenic or chemiluminescent substrates