Canine HMGB-1 (HighMobility Group Protein B1) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of HMGB-1 (HighMobility Group Protein B1) in canine samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Dog HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.