Canine Cys-C (Cystatin C) ELISA kit

This kit applies sandwich ELISA method for the quantitative detection of Cys-C (Cystatin C) in canine samples. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canine Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Canine Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Canine Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Canine Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.